Lipidomics Profiling

Lipid profilings is the analysis and identification of lipids in organisms, tissues or cells and the molecules with which they interact. In addition, the structure and function of lipids can also be understood, so as to reveal the relationship between lipid metabolism and the physiological and pathological processes of cells, organs and even the body. The contents of lipidomics research mainly include analysis and identification of lipids and their metabolites, lipid function and metabolic regulation, lipid metabolism pathways and networks.

The techniques of lipidomics research mainly include the extraction, separation, analysis and identification of lipids and the corresponding bioinformatics techniques. CPR mainly provides lipidomic analysis services based on biological mass spectrometry technology. The lipid analysis strategy based on mass spectrometry mainly includes liquid chromatography-mass spectrometry and shotgun lipidomics.

The liquid chromatography-mass spectrometry technology strategy is to use different lipid extraction methods to extract different types of lipids (lipid classes), or according to the difference in polarity of different types of lipids. Normal phase chromatography is used to classify the lipids of biological samples into different components at the species level. Then reverse phase chromatography is used to further separate the lipid molecules (molecular species) in the components, and then qualitative and quantitative analysis is performed using mass spectrometry.