Metagenome Studies
Shotgun Metagenome Seq
The method for shotgun metagenomic sequencing starts with the extraction of DNA from all cells in a community. The DNA is then fragmented into smaller components which are independently sequenced. This avoids using a specific genetic locus as a target for sequencing. Shotgun metagenomic sequencing is a relatively new environmental sequencing approach used to examine thousands of organisms in parallel and comprehensively sample all genes, providing insight into community biodiversity and function. Shotgun sequencing allows for the detection of low abundance members of microbial communities.
16S Amplicon Sequencing
Metagenomic studies are commonly performed by analyzing the prokaryotic 16S ribosomal RNA gene (16S rRNA), which is approximately 1,500 bp long and contains nine variable regions interspersed between conserved regions. Variable regions of 16S rRNA are frequently used in phylogenetic classifications such as genus or species in diverse microbial populations. This protocol describes a method for preparing samples for sequencing the variable V3 and V4 regions of the 16S rRNA gene. This protocol can also be used for sequencing other regions with different region‐specific primers.
Fungal Genome Sequencing
The first and possibly the most critical step in any genome project is the selection of organism to be sequenced based on medical, agricultural, or industrial significance, ecological diversity and phylogenetic breadth. With the use of short and long read sequencing we complete the genome sequencing, assembly and annotation of the entire fungal genome.
