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GUT MICROBIOTA TEST FOR LEAKY GUT SYNDROME

What is Leaky GUT:

Do you consistently suffer from a leaking gut? Well, it does not mean like a leaking pipe, but similar to that, when the lining in your gut becomes damaged, it allows substances to pass through. When the mucous lining of your intestines which is designed to absorb water and nutrients from food into the bloodstream gets compromised, you may suffer from this condition.

a leaky gut barrier creates larger holes in the lining, allowing bacteria, toxins, and undigested food particles to pass into the bloodstream, which can be dangerous for your health.

The toxins trigger inflammatory responses that cause various autoimmune and digestive diseases.

Leaky gut Syndrome Symptoms:

• If you suffer from frequent stomach aches, bloating, gas, constipation or diarrhoea leaky gut syndrome can be a key factor.

• According to studies, both adults and children suffering from digestive issues like constipation, mostly have increased intestinal permeability.

• If you suffer from a leaky gut, you may often find certain foods triggering a reaction by your immune system which leads to hives, swelling, wheezing, or digestive distress.

• One of the main symptom of issues with your gut is recurrent skin inflammation and disorders like acne, eczema, psoriasis, and even hives.

• Consistently joints Pain can also be a result of a leaky gut as harmful bacteria in the bloodstream activate inflammatory cells that can trigger and worsen pain and inflammation.

GUT MICROBIOTA Associated with LEAKY GUT

Several bacteria have been associated with “leaky gut,” a condition where the intestinal lining becomes permeable, allowing toxins, bacteria, and other substances to pass into the bloodstream.

The primary bacterial groups associated with leaky gut include:

1. Pathogenic Bacteria:

• Escherichia coli (E. coli): Certain strains of E. coli, particularly enteropathogenic and enterohemorrhagic types, can damage the intestinal lining and contribute to leaky gut.
• Salmonella: Known to cause food poisoning, it can damage the intestinal mucosa, leading to increased intestinal permeability.
• Clostridium difficile (C. difficile): A pathogen that can cause severe colitis and disrupts the intestinal barrier integrity.
• Helicobacter pylori (H. pylori): Commonly associated with stomach ulcers but can also impair gut lining function.

2. Opportunistic Bacteria:

• Klebsiella: Has been linked to ankylosing spondylitis and other autoimmune diseases, potentially contributing to intestinal permeability.
• Enterococcus faecalis: An opportunistic pathogen that can disrupt the intestinal barrier and contribute to inflammation.

3. Overgrowth of Gram-negative Bacteria:

• Gram-negative bacteria (like certain species of Bacteroides and Proteobacteria) produce lipopolysaccharides (LPS), a potent endotoxin that can trigger inflammation and promote leaky gut by compromising tight junctions in the intestinal epithelium.

4. Dysbiosis-Associated Bacteria:

• Desulfovibrio: A sulfate-reducing bacteria that produces hydrogen sulfide, which can damage the mucosal lining of the gut.
• Fusobacterium nucleatum: Associated with colorectal cancer and other inflammatory conditions, contributing to intestinal barrier dysfunction.

5. Bacteria Associated with Dysbiosis:

• A decrease in beneficial bacteria like Lactobacillus and Bifidobacterium can lead to dysbiosis, where harmful bacteria can proliferate and cause damage to the gut lining.

3. Novelgene’s approach:

Novelgene has developed an innovative approach to identify and address the underlying causes of leaky gut syndrome and gut dysbiosis. Our methodology involves a comprehensive analysis of the gut microbiota and their metabolic outputs to pinpoint the precise microbial and metabolic factors contributing to gut barrier dysfunction.

1. Development of Targeted Bacterial Panels:

We have curated specialized bacterial panels that specifically target and identify bacteria associated with leaky gut syndrome and gut dysbiosis. These panels are designed to detect both well-known pathogens and lesser-known bacteria that are present in low abundance but may have significant effects on gut health. This approach allows us to capture the full spectrum of microbial players involved in disrupting the intestinal barrier.

2. Innovative Tools and Methods for Low-Abundance Bacteria:

To ensure that even low-abundance bacteria, which may play crucial roles in leaky gut and dysbiosis, are accurately detected, Novelgene has developed state-of-the-art tools and methods. Our proprietary techniques enable us to selectively isolate and analyze these bacteria, providing a more detailed understanding of the microbial community dynamics and their contributions to gut health.

3. Development of Metabolite Panels:

In addition to bacterial panels, we have created specific metabolite panels to identify key metabolites linked to leaky gut syndrome. These metabolites are produced by the gut microbiota and can directly affect intestinal permeability and inflammation. Our panels are tailored to detect these metabolites in samples, providing a comprehensive view of the metabolic activities that may contribute to leaky gut.

4. Integrated Microbiota and Metabolite Analysis:

To gain a holistic understanding of the factors influencing leaky gut, we simultaneously analyze microbiota composition and metabolite profiles from the same samples. This integrated approach allows us to correlate microbial abundance with metabolite production and compare the results against data from healthy individuals. By establishing these correlations, we can identify specific microbial-metabolite interactions that may be responsible for gut barrier dysfunction.

5. Data Correlation and Comparative Studies:

Our analytical framework includes advanced bioinformatics and statistical methods to study correlations between the microbiota and metabolite data. These results are compared against data from healthy individuals to identify significant deviations that may be contributing to leaky gut. By mapping these deviations, we can pinpoint specific bacterial strains or metabolic pathways that are associated with the condition.

6. Designing Personalized Remedies:

Based on our comprehensive analysis of microbiota and metabolites, we can develop targeted and personalized remedies for individuals suffering from leaky gut syndrome. These remedies may include tailored probiotic and prebiotic formulations, dietary recommendations, and other therapeutic interventions designed to restore a healthy balance of gut bacteria and metabolites, ultimately improving gut barrier function and overall health.

Novelgene’s approach provides a cutting-edge, integrated method to not only understand the complexities of leaky gut and dysbiosis but also to offer personalized, data-driven solutions for individuals seeking to restore their gut health.

Why Choose Novelgene:

• Unique In-house Protocols: Novelgene has developed proprietary methodologies for gut microbiota analysis, starting from stool sample collection to final analysis.
• Careful Sample Collection: Extra care is taken during stool sample collection, with controlled temperature management and transportation in specially designed boxes.
• Temperature Control & Processing: Ambient temperature is maintained during transportation, and samples are immediately processed for DNA isolation, preserving natural microbial diversity.
• Optimized Procedures: The entire procedure, from DNA isolation to sequencing, has been optimized for speed, making it faster than other labs in India.
• Advanced Markers and Tools: Novelgene has developed specialized markers and tools to identify potential metabolites produced by the microbial community and analyze microbiota ratios, which may be linked to neurological disorders.
• Comprehensive Microbiota Reports: 2-3 week

• GUT-Microbiome Diversity Score
• F/B Ratio: Firmicutes and Bacteroidetes are two major classes of Bacteria, dominant in the human gut microbiome. The Firmicutes to Bacteroidetes ratio (F/B ) correlates with the balance or imbalance in the gut microbiome. An ideal F/B ratio would lie between 0.5 to 1.2. An imbalance may indicate a risk for metabolic disorders.
• Know your most abundant bacteria, Pathogenic bacteria, opportunistic bacteria, probiotic bacteria etc with their %abundance
• Microbiome-based metabolic indicators: Butyrate, Gas, inflammatory and non-inflammatory producers.
• Finding on the basis of DYSBIOSIS and Summary Reports.
• Phase I: GUT-REBOOT (Initially 3-4 weeks under the supervision of gastroenterologist and nutritionist)
• Phase 2: GUT-REBUILD (Next 3-4 weeks under the supervision of gastroenterologist and nutritionist)
• Phase 3: GUT-RETAIN (Next 4-6 weeks under the supervision of gastroenterologist and nutritionist)

Samples Requirement: STOOL SAMPLE ONLY (samples collection will be taken care by Novelgene Wellness Team only)

“Fecal samples, once regarded solely as waste, are increasingly recognized for their therapeutic potential in improving health outcomes”